› 3u15

X-ray diffraction
1.8Å resolution

Structure of hDMX with Dimer Inducing Indolyl Hydantoin RO-2443

Released:
DOI: 10.2210/pdb3u15/pdb
PDB entry 3u15 coloured by chain, front view.
PDB entry 3u15 coloured by chain, side view.
PDB entry 3u15 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Activation of the p53 pathway by small-molecule-induced MDM2 and MDMX dimerization.
Graves B, Thompson T, Xia M, Janson C, Lukacs C, Deo D, Di Lello P, Fry D, Garvie C, Huang KS, Gao L, Tovar C, Lovey A, Wanner J, Vassilev LT
Proc Natl Acad Sci U S A 109 11788-93 (2012)
PMID: 22745160

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127329 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein Mdm4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 100 amino acids
Theoretical weight: 11.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15151 (Residues: 14-111; Coverage: 20%)
Gene names: MDM4, MDMX
Structure domains: SWIB/MDM2 domain

Ligands and Environments

2 bound ligands:
Ligand 03M 4 x 03M
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P31
Unit cell:
a: 73.031Å b: 73.031Å c: 68.774Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.184 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

49 review citations

MDM2, MDMX and p53 in oncogenesis and cancer therapy.
Wade et al. (2013)
48 more

8 mentions without citation

Targeting p53-MDM2-MDMX loop for cancer therapy.
Zhang et al. (2014)
7 more