› 3u1n

X-ray diffraction
3.1Å resolution

Structure of the catalytic core of human SAMHD1

Released:
DOI: 10.2210/pdb3u1n/pdb
PDB entry 3u1n coloured by chain, front view.
PDB entry 3u1n coloured by chain, side view.
PDB entry 3u1n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
HIV-1 restriction factor SAMHD1 is a deoxynucleoside triphosphate triphosphohydrolase.
Goldstone DC, Ennis-Adeniran V, Hedden JJ, Groom HC, Rice GI, Christodoulou E, Walker PA, Kelly G, Haire LF, Yap MW, de Carvalho LP, Stoye JP, Crow YJ, Taylor IA, Webb M
Nature 480 379-82 (2011)
PMID: 22056990

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195221 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 528 amino acids
Theoretical weight: 61.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y3Z3 (Residues: 120-626; Coverage: 81%)
Gene names: MOP5, SAMHD1
Sequence domains: HD domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 4 x PO4
Ligand ZN 4 x ZN
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 81.516Å b: 95.818Å c: 96.661Å
α: 91.184° β: 109.238° γ: 115.201°
R-values:
R R work R free
0.197 0.196 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

164 review citations

Intrinsic antiviral immunity.
Yan et al. (2012)
163 more

15 mentions without citation

SAMHD1 Functions and Human Diseases.
Coggins et al. (2020)
14 more