› 3u2x

X-ray diffraction
1.77Å resolution

Crystal Structure of Human Glycogenin-1 (GYG1) complexed with manganese, UDP and 1'-deoxyglucose

Released:
DOI: 10.2210/pdb3u2x/pdb
PDB entry 3u2x coloured by chain, front view.
PDB entry 3u2x coloured by chain, side view.
PDB entry 3u2x coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Chaikuad A, Froese DS, Krysztofinska E, von Delft F, Weigelt J, Arrowsmith CH, Edwards AM, Bountra C, Oppermann U, Yue WW, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycogenin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 29.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46976 (Residues: 1-262; Coverage: 75%)
Gene names: GYG, GYG1
Sequence domains: Glycosyl transferase family 8
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand UDP 2 x UDP
Ligand ASO 2 x ASO
Ligand EDO 11 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P1
Unit cell:
a: 46.7Å b: 46.86Å c: 69.7Å
α: 79.57° β: 88.96° γ: 77.25°
R-values:
R R work R free
0.2 0.198 0.247
Expression system: Escherichia coli

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