› 3u31

X-ray diffraction
2.2Å resolution

Plasmodium falciparum Sir2A preferentially hydrolyzes medium and long chain fatty acyl lysine

Released:
DOI: 10.2210/pdb3u31/pdb
PDB entry 3u31 coloured by chain, front view.
PDB entry 3u31 coloured by chain, side view.
PDB entry 3u31 coloured by chain, top view.
Source organisms:
Primary publication:
Plasmodium falciparum Sir2A preferentially hydrolyzes medium and long chain fatty acyl lysine.
Zhu AY, Zhou Y, Khan S, Deitsch KW, Hao Q, Lin H
ACS Chem Biol 7 155-9 (2012)
PMID: 21992006

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159419 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD-dependent protein deacylase Sir2A Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 32.05 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IE47 (Residues: 1-273; Coverage: 100%)
Gene names: PF13_0152, PF3D7_1328800, Sir2, Sir2A
Sequence domains: Sir2 family
Structure domains:
Histone H3.1 Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.62 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 5-14; Coverage: 7%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

3 bound ligands:
Ligand NAD 1 x NAD
Ligand GOL 1 x GOL
Ligand ZN 1 x ZN
1 modified residue:
Ligand MYK 1 x MYK

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P21212
Unit cell:
a: 32.173Å b: 102.734Å c: 105.184Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.246
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Protein lysine acylation and cysteine succination by intermediates of energy metabolism.
Lin et al. (2012)
9 more

6 mentions without citation

Modulation of epigenetic targets for anticancer therapy: clinicopathological relevance, structural data and drug discovery perspectives.
Andreoli et al. (2013)
5 more