› 3u7x

X-ray diffraction
2.1Å resolution

Crystal structure of the human eIF4E-4EBP1 peptide complex without cap

Released:
DOI: 10.2210/pdb3u7x/pdb
PDB entry 3u7x coloured by chain, front view.
PDB entry 3u7x coloured by chain, side view.
PDB entry 3u7x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the allosteric effects of 4EBP1 on the eukaryotic translation initiation factor eIF4E.
Siddiqui N, Tempel W, Nedyalkova L, Volpon L, Wernimont AK, Osborne MJ, Park HW, Borden KL
J Mol Biol 415 781-92 (2012)
PMID: 22178476

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-139176 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 4E Chains: A, B
Molecule details ›
Chains: A, B
Length: 217 amino acids
Theoretical weight: 25.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06730 (Residues: 1-217; Coverage: 100%)
Gene names: EIF4E, EIF4EL1, EIF4F
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e
Eukaryotic translation initiation factor 4E-binding protein 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 20 amino acids
Theoretical weight: 2.36 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q13541 (Residues: 47-66; Coverage: 17%)
Gene name: EIF4EBP1

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand UNX 20 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 86.5Å b: 38.263Å c: 93.533Å
α: 90° β: 97.97° γ: 90°
R-values:
R R work R free
0.199 0.198 0.224
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

Eukaryotic initiation factor 4E-binding protein 1 (4E-BP1): a master regulator of mRNA translation involved in tumorigenesis.
Musa et al. (2016)
6 more

5 mentions without citation

Control of the eIF4E activity: structural insights and pharmacological implications.
Romagnoli et al. (2021)
4 more