› 3u9c

X-ray diffraction
3.2Å resolution

Structure of a C-terminal deletion mutant of human protein kinase CK2 catalytic subunit with the ATP-competitive inhibitor resorufin

Released:
DOI: 10.2210/pdb3u9c/pdb
PDB entry 3u9c coloured by chain, front view.
PDB entry 3u9c coloured by chain, side view.
PDB entry 3u9c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Low-density crystal packing of human protein kinase CK2 catalytic subunit in complex with resorufin or other ligands: a tool to study the unique hinge-region plasticity of the enzyme without packing bias.
Klopffleisch K, Issinger OG, Niefind K
Acta Crystallogr D Biol Crystallogr 68 883-92 (2012)
PMID: 22868753

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-159383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Casein kinase II subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 40.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P68400 (Residues: 1-335; Coverage: 86%)
Gene names: CK2A1, CSNK2A1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 7 x SO4
Ligand 04G 2 x 04G
Ligand GOL 5 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43212
Unit cell:
a: 127.777Å b: 127.777Å c: 125.766Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.231
Expression system: Escherichia coli

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Citations

1 review citation

Role of Computational Methods in Going beyond X-ray Crystallography to Explore Protein Structure and Dynamics.
Srivastava et al. (2018)
9 other articles

2 mentions without citation

The right angle (RA) motif: a prevalent ribosomal RNA structural pattern found in group I introns.
Grabow et al. (2012)
1 more