› 3u9u

X-ray diffraction
3.42Å resolution

Crystal Structure of Extracellular Domain of Human ErbB4/Her4 in complex with the Fab fragment of mAb1479

Released:
DOI: 10.2210/pdb3u9u/pdb
PDB entry 3u9u coloured by chain, front view.
PDB entry 3u9u coloured by chain, side view.
PDB entry 3u9u coloured by chain, top view.
Source organisms:
Primary publication:
Proteolytic processing of ErbB4 in breast cancer.
Hollmén M, Liu P, Kurppa K, Wildiers H, Reinvall I, Vandorpe T, Smeets A, Deraedt K, Vahlberg T, Joensuu H, Leahy DJ, Schöffski P, Elenius K
PLoS One 7 e39413 (2012)
PMID: 22761786

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-213418 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab Heavy Chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 224 amino acids
Theoretical weight: 24.2 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab Light Chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 219 amino acids
Theoretical weight: 24.21 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Receptor tyrosine-protein kinase erbB-4 Chains: E, F
Molecule details ›
Chains: E, F
Length: 625 amino acids
Theoretical weight: 69.87 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q15303 (Residues: 26-650; Coverage: 49%)
Gene names: ERBB4, HER4
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 83.121Å b: 110.914Å c: 362.051Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.234 0.272
Expression systems:
  • Mus musculus
  • Cricetulus griseus

Quick links

Citations

6 review citations

Comprehensive profiling of EGFR/HER receptors for personalized treatment of gynecologic cancers.
Reyes et al. (2014)
5 more

5 mentions without citation

Structural Basis for Recognition of Human Enterovirus 71 by a Bivalent Broadly Neutralizing Monoclonal Antibody.
Ye et al. (2016)
4 more