› 3uan

X-ray diffraction
1.84Å resolution

Crystal structure of 3-O-sulfotransferase (3-OST-1) with bound PAP and heptasaccharide substrate

Released:
DOI: 10.2210/pdb3uan/pdb
PDB entry 3uan coloured by chain, front view.
PDB entry 3uan coloured by chain, side view.
PDB entry 3uan coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Dissecting the substrate recognition of 3-O-sulfotransferase for the biosynthesis of anticoagulant heparin.
Moon AF, Xu Y, Woody SM, Krahn JM, Linhardt RJ, Liu J, Pedersen LC
Proc Natl Acad Sci U S A 109 5265-70 (2012)
PMID: 22431632

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128671 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Heparan sulfate glucosamine 3-O-sulfotransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 31.4 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O35310 (Residues: 48-311; Coverage: 91%)
Gene names: 3ost, 3ost1, Hs3st1
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

Carbohydrate polymer : NEW Components: BDP, SGN, IDS, NGY
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BDP, SGN, IDS, NGY
Carbohydrate polymer
2 bound ligands:
Ligand A3P 2 x A3P
Ligand BDP 1 x BDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 52.883Å b: 62.168Å c: 86.373Å
α: 90° β: 95.16° γ: 90°
R-values:
R R work R free
0.175 0.174 0.202
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Demystifying heparan sulfate-protein interactions.
Xu et al. (2014)
10 more

12 mentions without citation

Heparan sulfate 3-O-sulfation: a rare modification in search of a function.
Thacker et al. (2014)
11 more