› 3uem

X-ray diffraction
2.29Å resolution

Crystal structure of human PDI bb'a' domains

Released:
DOI: 10.2210/pdb3uem/pdb
PDB entry 3uem coloured by chain, front view.
PDB entry 3uem coloured by chain, side view.
PDB entry 3uem coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human protein-disulfide isomerase is a redox-regulated chaperone activated by oxidation of domain a'.
Wang C, Yu J, Huo L, Wang L, Feng W, Wang CC
J Biol Chem 287 1139-49 (2012)
PMID: 22090031

Function and Biology Details

Reaction catalysed: 
Catalyzes the rearrangement of -S-S- bonds in proteins
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein disulfide-isomerase Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 40.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07237 (Residues: 137-479; Coverage: 70%)
Gene names: ERBA2L, P4HB, PDI, PDIA1, PO4DB
Sequence domains:
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand D1D 1 x D1D
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 63.585Å b: 76.076Å c: 79.656Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.28
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Endoplasmic Reticulum Stress and Associated ROS.
Zeeshan et al. (2016)
23 more

12 mentions without citation

New targets for HIV drug discovery.
Puhl et al. (2019)
11 more