› 3uen

X-ray diffraction
1.9Å resolution

Crystal structure of TopBP1 BRCT4/5 domains

Released:
DOI: 10.2210/pdb3uen/pdb
PDB entry 3uen coloured by chain, front view.
PDB entry 3uen coloured by chain, side view.
PDB entry 3uen coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into recognition of MDC1 by TopBP1 in DNA replication checkpoint control.
Leung CC, Sun L, Gong Z, Burkat M, Edwards R, Assmus M, Chen J, Glover JN
Structure 21 1450-9 (2013)
PMID: 23891287

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 2-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 22.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92547 (Residues: 549-746; Coverage: 13%)
Gene names: KIAA0259, TOPBP1
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain

Ligands and Environments

2 bound ligands:
Ligand SCN 1 x SCN
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P2221
Unit cell:
a: 35.902Å b: 48.801Å c: 126.088Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.224
Expression system: Escherichia coli

Quick links

Citations

5 review citations

DNA damage kinase signaling: checkpoint and repair at 30 years.
Lanz et al. (2019)
4 more

3 mentions without citation

Phosphorylation-mediated interactions with TOPBP1 couple 53BP1 and 9-1-1 to control the G1 DNA damage checkpoint.
Bigot et al. (2019)
2 more