› 3uit

X-ray diffraction
2.05Å resolution

Overall structure of Patj/Pals1/Mals complex

Released:
DOI: 10.2210/pdb3uit/pdb
PDB entry 3uit coloured by chain, front view.
PDB entry 3uit coloured by chain, side view.
PDB entry 3uit coloured by chain, top view.
Source organisms:
Primary publication:
Structure of an L27 domain heterotrimer from cell polarity complex Patj/Pals1/Mals2 reveals mutually independent L27 domain assembly mode.
Zhang J, Yang X, Wang Z, Zhou H, Xie X, Shen Y, Long J
J Biol Chem 287 11132-40 (2012)
PMID: 22337881

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123312 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
InaD-like protein; Protein PALS1; Protein lin-7 homolog B Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 265 amino acids
Theoretical weight: 29.86 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: F1MAD2 (Residues: 1-68; Coverage: 4%)
  • Canonical: Q8N3R9 (Residues: 119-232; Coverage: 17%)
  • Canonical: O88951 (Residues: 3-66; Coverage: 31%)
Gene names: Cipp, Inadl, Lin7b, MPP5, Mals2, PALS1, Patj, Veli2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ACT 20 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6122
Unit cell:
a: 145.194Å b: 145.194Å c: 202.547Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.18 0.22
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The Mammalian Blood-Testis Barrier: Its Biology and Regulation.
Mruk et al. (2015)
3 more

2 mentions without citation

Structure of an L27 domain heterotrimer from cell polarity complex Patj/Pals1/Mals2 reveals mutually independent L27 domain assembly mode.
Zhang et al. (2012)
1 more