› 3uky

X-ray diffraction
2.35Å resolution

Mouse importin alpha: yeast CBP80 cNLS complex

Released:
DOI: 10.2210/pdb3uky/pdb
PDB entry 3uky coloured by chain, front view.
PDB entry 3uky coloured by chain, side view.
PDB entry 3uky coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of high-affinity nuclear localization signal interactions with importin-α.
Marfori M, Lonhienne TG, Forwood JK, Kobe B
Traffic 13 532-48 (2012)
PMID: 22248489

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152691 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 510 amino acids
Theoretical weight: 55.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Nuclear cap-binding protein complex subunit 1 Chain: C
Molecule details ›
Chain: C
Length: 32 amino acids
Theoretical weight: 4.08 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P34160 (Residues: 1-30; Coverage: 4%)
Gene names: CBC1, CBP80, GCR3, STO1, SUT1, YM8564.07, YM9553.01, YMR125W

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 78.195Å b: 89.747Å c: 98.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.215
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Broad targeting of resistance to apoptosis in cancer.
Mohammad et al. (2015)
8 more

5 mentions without citation

Nuclear Localization of the DNA Repair Scaffold XRCC1: Uncovering the Functional Role of a Bipartite NLS.
Kirby et al. (2015)
4 more