› 3ul1

X-ray diffraction
1.9Å resolution

Mouse importin alpha: nucleoplasmin cNLS peptide complex

Released:
DOI: 10.2210/pdb3ul1/pdb
PDB entry 3ul1 coloured by chain, front view.
PDB entry 3ul1 coloured by chain, side view.
PDB entry 3ul1 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of high-affinity nuclear localization signal interactions with importin-α.
Marfori M, Lonhienne TG, Forwood JK, Kobe B
Traffic 13 532-48 (2012)
PMID: 22248489

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138599 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 510 amino acids
Theoretical weight: 55.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Nucleoplasmin Chain: A
Molecule details ›
Chain: A
Length: 22 amino acids
Theoretical weight: 2.29 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P05221 (Residues: 153-172; Coverage: 10%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 79.156Å b: 90.094Å c: 99.332Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.222
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Broad targeting of resistance to apoptosis in cancer.
Mohammad et al. (2015)
8 more

10 mentions without citation

Structure of importin-α bound to a non-classical nuclear localization signal of the influenza A virus nucleoprotein.
Nakada et al. (2015)
9 more