› 3un0

X-ray diffraction
2.3Å resolution

Crystal Structure of MDC1 FHA Domain

Released:
DOI: 10.2210/pdb3un0/pdb
PDB entry 3un0 coloured by chain, front view.
PDB entry 3un0 coloured by chain, side view.
PDB entry 3un0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The molecular basis of ATM-dependent dimerization of the Mdc1 DNA damage checkpoint mediator.
Jungmichel S, Clapperton JA, Lloyd J, Hari FJ, Spycher C, Pavic L, Li J, Haire LF, Bonalli M, Larsen DH, Lukas C, Lukas J, MacMillan D, Nielsen ML, Stucki M, Smerdon SJ
Nucleic Acids Res 40 3913-28 (2012)
PMID: 22234878

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172077 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mediator of DNA damage checkpoint protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 115 amino acids
Theoretical weight: 12.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q14676 (Residues: 26-138; Coverage: 5%)
Gene names: KIAA0170, MDC1, NFBD1
Sequence domains: FHA domain
Structure domains: Tumour Suppressor Smad4

Ligands and Environments

1 bound ligand:
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 35.25Å b: 54.711Å c: 98.063Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.261
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

ATM, ATR, and DNA-PK: The Trinity at the Heart of the DNA Damage Response.
Blackford et al. (2017)
10 more

1 mention without citation

The molecular basis of ATM-dependent dimerization of the Mdc1 DNA damage checkpoint mediator.
Jungmichel et al. (2012)