› 3unw

X-ray diffraction
2.56Å resolution

Crystal Structure of Human GAC in Complex with Glutamate

Released:
DOI: 10.2210/pdb3unw/pdb
PDB entry 3unw coloured by chain, front view.
PDB entry 3unw coloured by chain, side view.
PDB entry 3unw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Full-length human glutaminase in complex with an allosteric inhibitor.
DeLaBarre B, Gross S, Fang C, Gao Y, Jha A, Jiang F, Song J J, Wei W, Hurov JB
Biochemistry 50 10764-70 (2011)
PMID: 22049910

Function and Biology Details

Reaction catalysed: 
L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131797 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaminase kidney isoform, mitochondrial 65 kDa chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 534 amino acids
Theoretical weight: 58.94 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O94925 (Residues: 71-600; Coverage: 79%)
Gene names: GLS, GLS1, KIAA0838
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GLU 4 x GLU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 50.66Å b: 139.614Å c: 178.385Å
α: 90° β: 94.14° γ: 90°
R-values:
R R work R free
0.228 0.226 0.261
Expression system: Not provided

Quick links

Citations

26 review citations

From Krebs to clinic: glutamine metabolism to cancer therapy.
Altman et al. (2016)
25 more

2 mentions without citation

A tale of two glutaminases: homologous enzymes with distinct roles in tumorigenesis.
Katt et al. (2017)
1 more