› 3uot

X-ray diffraction
1.8Å resolution

Crystal Structure of MDC1 FHA Domain in Complex with a Phosphorylated Peptide from the MDC1 N-terminus

Released:
DOI: 10.2210/pdb3uot/pdb
PDB entry 3uot coloured by chain, front view.
PDB entry 3uot coloured by chain, side view.
PDB entry 3uot coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The molecular basis of ATM-dependent dimerization of the Mdc1 DNA damage checkpoint mediator.
Jungmichel S, Clapperton JA, Lloyd J, Hari FJ, Spycher C, Pavic L, Li J, Haire LF, Bonalli M, Larsen DH, Lukas C, Lukas J, MacMillan D, Nielsen ML, Stucki M, Smerdon SJ
Nucleic Acids Res 40 3913-28 (2012)
PMID: 22234878

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172075 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mediator of DNA damage checkpoint protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 13.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q14676 (Residues: 19-138; Coverage: 6%)
Gene names: KIAA0170, MDC1, NFBD1
Sequence domains: FHA domain
Structure domains: Tumour Suppressor Smad4
Mediator of DNA damage checkpoint protein 1 Chains: D, E
Molecule details ›
Chains: D, E
Length: 10 amino acids
Theoretical weight: 1.41 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q14676 (Residues: 1-10; Coverage: 1%)
Gene names: KIAA0170, MDC1, NFBD1

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 4 x MSE
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX10.1
Spacegroup: P212121
Unit cell:
a: 58.485Å b: 59.88Å c: 72.081Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.243
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

11 review citations

The ATM protein kinase: regulating the cellular response to genotoxic stress, and more.
Shiloh et al. (2013)
10 more

2 mentions without citation

The molecular basis of ATM-dependent dimerization of the Mdc1 DNA damage checkpoint mediator.
Jungmichel et al. (2012)
1 more