› 3upc

X-ray diffraction
2.8Å resolution

A general strategy for the generation of human antibody variable domains with increased aggregation resistance

Released:
DOI: 10.2210/pdb3upc/pdb
PDB entry 3upc coloured by chain, front view.
PDB entry 3upc coloured by chain, side view.
PDB entry 3upc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
General strategy for the generation of human antibody variable domains with increased aggregation resistance.
Dudgeon K, Rouet R, Kokmeijer I, Schofield P, Stolp J, Langley D, Stock D, Christ D
Proc Natl Acad Sci U S A 109 10879-84 (2012)
PMID: 22745168

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134595 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin heavy variable 3-23 Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 113 amino acids
Theoretical weight: 12.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01764 (Residues: 22-117; Coverage: 98%)
Gene name: IGHV3-23
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

4 bound ligands:
Ligand 1PE 2 x 1PE
Ligand PGE 1 x PGE
Ligand PEG 1 x PEG
Ligand PG4 3 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21212
Unit cell:
a: 82.61Å b: 143.12Å c: 145.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.247
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Therapeutic protein aggregation: mechanisms, design, and control.
Roberts CJ. (2014)
26 more

2 mentions without citation

General strategy for the generation of human antibody variable domains with increased aggregation resistance.
Dudgeon et al. (2012)
1 more