› 3us1

X-ray diffraction
2.8Å resolution

Structure of p63 DNA Binding Domain in Complex with a 22 Base Pair Response Element Containing a Two Base Pair "GC" Spacer Between Half Sites

Released:
DOI: 10.2210/pdb3us1/pdb
PDB entry 3us1 coloured by chain, front view.
PDB entry 3us1 coloured by chain, side view.
PDB entry 3us1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Pliable DNA conformation of response elements bound to transcription factor p63.
Chen C, Gorlatova N, Herzberg O
J Biol Chem 287 7477-86 (2012)
PMID: 22247550

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117004 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Tumor protein 63 Chains: A, D
Molecule details ›
Chains: A, D
Length: 203 amino acids
Theoretical weight: 22.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H3D4 (Residues: 166-362; Coverage: 29%)
Gene names: KET, P63, P73H, P73L, TP63, TP73L
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
5'-D(*AP*AP*AP*CP*AP*TP*GP*TP*TP*TP*GP*CP*AP*AP*AP*CP*AP*TP*GP*TP*TP*T)-3' Chain: F
Molecule details ›
Chain: F
Length: 22 nucleotides
Theoretical weight: 6.75 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 84.954Å b: 101.22Å c: 71.501Å
α: 90° β: 122.78° γ: 90°
R-values:
R R work R free
0.204 0.202 0.234
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Roles of p53 Family Structure and Function in Non-Canonical Response Element Binding and Activation.
Cai et al. (2019)
1 more

4 mentions without citation

Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9.
McLellan et al. (2011)
3 more