› 3uv5

X-ray diffraction
2.03Å resolution

Crystal Structure of the tandem bromodomains of human Transcription initiation factor TFIID subunit 1 (TAF1)

Released:
DOI: 10.2210/pdb3uv5/pdb
PDB entry 3uv5 coloured by chain, front view.
PDB entry 3uv5 coloured by chain, side view.
PDB entry 3uv5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Histone recognition and large-scale structural analysis of the human bromodomain family.
Filippakopoulos P, Picaud S, Mangos M, Keates T, Lambert JP, Barsyte-Lovejoy D, Felletar I, Volkmer R, Müller S, Pawson T, Gingras AC, Arrowsmith CH, Knapp S
Cell 149 214-31 (2012)
PMID: 22464331

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149352 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription initiation factor TFIID subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 30.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21675 (Residues: 1394-1656; Coverage: 14%)
Gene names: BA2R, CCG1, CCGS, TAF1, TAF2A
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2221
Unit cell:
a: 57.76Å b: 199.99Å c: 88.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.23
Expression system: Escherichia coli BL21(DE3)

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Citations

245 review citations

Modification of enhancer chromatin: what, how, and why?
Calo et al. (2013)
244 more

5 mentions without citation

Histone recognition and large-scale structural analysis of the human bromodomain family.
Filippakopoulos et al. (2012)
4 more