3uvu

X-ray diffraction
2.38Å resolution

Structural basis of nuclear import of Flap endonuclease 1 (FEN1)

Released:
Source organisms:
Primary publication:
Structural basis of nuclear import of flap endonuclease 1 (FEN1).
Acta Crystallogr D Biol Crystallogr 68 743-50 (2012)
PMID: 22751659

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-153939 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Flap endonuclease 1 Chain: B
Molecule details ›
Chain: B
Length: 19 amino acids
Theoretical weight: 2.11 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P39748 (Residues: 352-370; Coverage: 5%)
Gene names: FEN1, RAD2
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 510 amino acids
Theoretical weight: 55.33 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52293 (Residues: 70-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 78.87Å b: 89.561Å c: 100.343Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.215
Expression systems:
  • Not provided
  • Escherichia coli