› 3uzs

X-ray diffraction
4.52Å resolution

Structure of the C13.28 RNA Aptamer Bound to the G Protein-Coupled Receptor Kinase 2-Heterotrimeric G Protein Beta 1 and Gamma 2 Subunit Complex

Released:
DOI: 10.2210/pdb3uzs/pdb
PDB entry 3uzs coloured by chain, front view.
PDB entry 3uzs coloured by chain, side view.
PDB entry 3uzs coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Molecular mechanism for inhibition of g protein-coupled receptor kinase 2 by a selective RNA aptamer.
Tesmer VM, Lennarz S, Mayer G, Tesmer JJ
Structure 20 1300-9 (2012)
PMID: 22727813

Function and Biology Details

Reaction catalysed: 
ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-149113 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
Beta-adrenergic receptor kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 689 amino acids
Theoretical weight: 79.75 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P21146 (Residues: 1-689; Coverage: 100%)
Gene names: ADRBK1, GRK2
Sequence domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.42 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62871 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 74 amino acids
Theoretical weight: 8.41 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63212 (Residues: 1-67; Coverage: 94%)
Gene name: GNG2
Sequence domains: GGL domain
C13.28 RNA Aptamer Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 9.08 KDa

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand CMT 1 x CMT

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P312
Unit cell:
a: 257.277Å b: 257.277Å c: 99.429Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.184 0.268
Expression systems:
  • Spodoptera frugiperda
  • Trichoplusia ni

Quick links

Citations

18 review citations

Embracing proteins: structural themes in aptamer-protein complexes.
Gelinas et al. (2016)
17 more

5 mentions without citation

Structural insights into G protein-coupled receptor kinase function.
Homan et al. (2014)
4 more