› 3v00

X-ray diffraction
2.9Å resolution

Studies of a constitutively active G-alpha subunit provide insights into the mechanism of G protein activation.

Released:
DOI: 10.2210/pdb3v00/pdb
PDB entry 3v00 coloured by chain, front view.
PDB entry 3v00 coloured by chain, side view.
PDB entry 3v00 coloured by chain, top view.
Source organisms:
Primary publication:
A constitutively active Gα subunit provides insights into the mechanism of G protein activation.
Singh G, Ramachandran S, Cerione RA
Biochemistry 51 3232-40 (2012)
PMID: 22448927

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
homo trimer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-138149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(t) subunit alpha-1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 356 amino acids
Theoretical weight: 41 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10824 (Residues: 1-2, 220-247, 256-299; Coverage: 21%)
  • Canonical: P04695 (Residues: 3-215, 244-251, 296-350; Coverage: 79%)
Gene names: GNAT1, Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GDP 3 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P43212
Unit cell:
a: 93.173Å b: 93.173Å c: 380.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Structural mechanism of G protein activation by G protein-coupled receptor.
Duc et al. (2015)
5 other articles

2 mentions without citation

A constitutively active Gα subunit provides insights into the mechanism of G protein activation.
Singh et al. (2012)
1 more