› 3v1g

X-ray diffraction
2.2Å resolution

Forestalling insulin fibrillation by insertion of a chiral clamp mechanism-based application of protein engineering to global health

Released:
DOI: 10.2210/pdb3v1g/pdb
PDB entry 3v1g coloured by chain, front view.
PDB entry 3v1g coloured by chain, side view.
PDB entry 3v1g coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Wan ZL, Hua QX, Wickramasinghe NP, Huang K, Petkova AT, Hu SQ, Phillips NB, Yeh IJ, Whittake J, Ismail-Beigi F, Katsoyyannis PG, Tycko R, Weiss MA

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134235 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand IPH 1 x IPH
Ligand CL 1 x CL
1 modified residue:
Ligand DGL 2 x DGL

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: R3
Unit cell:
a: 79.081Å b: 79.081Å c: 37.402Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.229 0.323
Expression system: Not provided

Quick links