› 3v5r

X-ray diffraction
2.1Å resolution

Crystal structure of the unliganded form of Gal3p

Released:
DOI: 10.2210/pdb3v5r/pdb
PDB entry 3v5r coloured by chain, front view.
PDB entry 3v5r coloured by chain, side view.
PDB entry 3v5r coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.
Lavy T, Kumar PR, He H, Joshua-Tor L
Genes Dev 26 294-303 (2012)
PMID: 22302941

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-146443 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein GAL3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 505 amino acids
Theoretical weight: 56.51 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P13045 (Residues: 17-520; Coverage: 97%)
Gene names: GAL3, YD8119.14, YDR009W
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 27 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3221
Unit cell:
a: 153.359Å b: 153.359Å c: 118.591Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.17 0.202
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Nutrient sensing and signaling in the yeast Saccharomyces cerevisiae.
Conrad et al. (2014)
4 more

1 mention without citation

The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.
Lavy et al. (2012)