› 3v6l

X-ray diffraction
2.2Å resolution

Crystal Structure of caspase-6 inactivation mutation

Released:
DOI: 10.2210/pdb3v6l/pdb
PDB entry 3v6l coloured by chain, front view.
PDB entry 3v6l coloured by chain, side view.
PDB entry 3v6l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inhibitory mechanism of caspase-6 phosphorylation revealed by crystal structures, molecular dynamics simulations, and biochemical assays.
Cao Q, Wang XJ, Liu CW, Liu DF, Li LF, Gao YQ, Su XD
J Biol Chem 287 15371-9 (2012)
PMID: 22433863

Function and Biology Details

Reaction catalysed: 
Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-|-
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157270 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Caspase-6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 282 amino acids
Theoretical weight: 32.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55212 (Residues: 21-293; Coverage: 93%)
Gene names: CASP6, MCH2
Sequence domains: Caspase domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P6522
Unit cell:
a: 126.35Å b: 126.35Å c: 165.82Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.169 0.202
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Activation and regulation of caspase-6 and its role in neurodegenerative diseases.
Wang et al. (2015)
5 more

2 mentions without citation

Inhibitory mechanism of caspase-6 phosphorylation revealed by crystal structures, molecular dynamics simulations, and biochemical assays.
Cao et al. (2012)
1 more