› 3v8x

X-ray diffraction
2.6Å resolution

The crystal structure of transferrin binding protein A (TbpA) from Neisserial meningitidis serogroup B in complex with full length human transferrin

Released:
DOI: 10.2210/pdb3v8x/pdb
PDB entry 3v8x coloured by chain, front view.
PDB entry 3v8x coloured by chain, side view.
PDB entry 3v8x coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for iron piracy by pathogenic Neisseria.
Noinaj N, Easley NC, Oke M, Mizuno N, Gumbart J, Boura E, Steere AN, Zak O, Aisen P, Tajkhorshid E, Evans RW, Gorringe AR, Mason AB, Steven AC, Buchanan SK
Nature 483 53-8 (2012)
PMID: 22327295

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136225 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Transferrin-binding protein A Chain: A
Molecule details ›
Chain: A
Length: 904 amino acids
Theoretical weight: 101.21 KDa
Source organism: Neisseria meningitidis serogroup B
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K0U9 (Residues: 25-915; Coverage: 100%)
Gene names: NMB0461, tbp1, tbpA
Sequence domains:
Structure domains:
Serotransferrin Chain: B
Molecule details ›
Chain: B
Length: 698 amino acids
Theoretical weight: 77.15 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P02787 (Residues: 1-698; Coverage: 100%)
Gene names: PRO1400, TF
Sequence domains: Transferrin
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, SIA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, SIA
Carbohydrate polymer
1 bound ligand:
Ligand C8E 2 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 91.014Å b: 129.362Å c: 198.589Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.267
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

41 review citations

Iron in infection and immunity.
Cassat et al. (2013)
40 more

17 mentions without citation

The transferrin-iron import system from pathogenic Neisseria species.
Noinaj et al. (2012)
16 more