› 3vd8

X-ray diffraction
2.07Å resolution

Crystal structure of human AIM2 PYD domain with MBP fusion

Released:
DOI: 10.2210/pdb3vd8/pdb
PDB entry 3vd8 coloured by chain, front view.
PDB entry 3vd8 coloured by chain, side view.
PDB entry 3vd8 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the absent in melanoma 2 (AIM2) pyrin domain provides insights into the mechanisms of AIM2 autoinhibition and inflammasome assembly.
Jin T, Perry A, Smith P, Jiang J, Xiao TS
J Biol Chem 288 13225-35 (2013)
PMID: 23530044

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127178 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Interferon-inducible protein AIM2; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 489 amino acids
Theoretical weight: 53.97 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEY0 (Residues: 27-384; Coverage: 97%)
  • Canonical: O14862 (Residues: 1-107; Coverage: 31%)
Gene names: AIM2, ECs5017, Z5632, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand EDO 4 x EDO
Ligand K 4 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 61.599Å b: 91.934Å c: 100.281Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

55 review citations

Inflammasomes: mechanism of action, role in disease, and therapeutics.
Guo et al. (2015)
54 more

23 mentions without citation

Structure, Activation and Regulation of NLRP3 and AIM2 Inflammasomes.
Sharma et al. (2021)
22 more