› 3vfo

X-ray diffraction
1.7Å resolution

crystal structure of HLA B*3508 LPEP157A, HLA mutant Ala157

Released:
DOI: 10.2210/pdb3vfo/pdb
PDB entry 3vfo coloured by chain, front view.
PDB entry 3vfo coloured by chain, side view.
PDB entry 3vfo coloured by chain, top view.
Source organisms:
Primary publication:
The energetic basis underpinning T-cell receptor recognition of a super-bulged peptide bound to a major histocompatibility complex class I molecule.
Liu YC, Chen Z, Burrows SR, Purcell AW, McCluskey J, Rossjohn J, Gras S
J Biol Chem 287 12267-76 (2012)
PMID: 22343629

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-111517 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5MK56 (Residues: 25-300; Coverage: 82%)
Gene name: HLA-B
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Lytic switch protein BZLF1 Chain: C
Molecule details ›
Chain: C
Length: 13 amino acids
Theoretical weight: 1.43 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
UniProt:
  • Canonical: P03206 (Residues: 52-64; Coverage: 5%)
Gene name: BZLF1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 50.99Å b: 81.52Å c: 110.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.246 0.279
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
5 more