› 3vh6

X-ray diffraction
3.35Å resolution

Crystal structure of the chicken CENP-T histone fold/CENP-W/CENP-S/CENP-X heterotetrameric complex, crystal form II

Released:
DOI: 10.2210/pdb3vh6/pdb
PDB entry 3vh6 coloured by chain, front view.
PDB entry 3vh6 coloured by chain, side view.
PDB entry 3vh6 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold.
Nishino T, Takeuchi K, Gascoigne KE, Suzuki A, Hori T, Oyama T, Morikawa K, Cheeseman IM, Fukagawa T
Cell 148 487-501 (2012)
PMID: 22304917

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-122768 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Centromere protein S Chain: A
Molecule details ›
Chain: A
Length: 140 amino acids
Theoretical weight: 15.56 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: E1BSW7 (Residues: 2-139; Coverage: 99%)
Gene names: APITD1, CENPS
Sequence domains: CENP-S protein
Structure domains: Histone, subunit A
Centromere protein X Chain: D
Molecule details ›
Chain: D
Length: 81 amino acids
Theoretical weight: 9.36 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DJH7 (Residues: 1-80; Coverage: 100%)
Gene names: CENPX, STRA13
Sequence domains: CENP-S associating Centromere protein X
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Centromere protein T Chain: T
Molecule details ›
Chain: T
Length: 111 amino acids
Theoretical weight: 12.68 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: F1NPG5 (Residues: 531-639; Coverage: 17%)
Gene name: CENPT
Sequence domains: Centromere kinetochore component CENP-T histone fold
Structure domains: Histone, subunit A
Centromere protein W Chain: W
Molecule details ›
Chain: W
Length: 77 amino acids
Theoretical weight: 8.86 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DJH6 (Residues: 1-76; Coverage: 100%)
Gene name: CENPW
Sequence domains: CENP-W protein
Structure domains: Histone, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: I213
Unit cell:
a: 158.51Å b: 158.51Å c: 158.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.211 0.273
Expression system: Escherichia coli

Quick links

Citations

47 review citations

The molecular basis for centromere identity and function.
McKinley et al. (2016)
46 more

2 mentions without citation

The CENP-A nucleosome: a dynamic structure and role at the centromere.
Quénet et al. (2012)
1 more