› 3vow

X-ray diffraction
2.15Å resolution

Crystal Structure of the Human APOBEC3C having HIV-1 Vif-binding Interface

Released:
DOI: 10.2210/pdb3vow/pdb
PDB entry 3vow coloured by chain, front view.
PDB entry 3vow coloured by chain, side view.
PDB entry 3vow coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The APOBEC3C crystal structure and the interface for HIV-1 Vif binding.
Kitamura S, Ode H, Nakashima M, Imahashi M, Naganawa Y, Kurosawa T, Yokomaku Y, Yamane T, Watanabe N, Suzuki A, Sugiura W, Iwatani Y
Nat Struct Mol Biol 19 1005-10 (2012)
PMID: 23001005

Function and Biology Details

Reaction catalysed: 
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA dC->dU-editing enzyme APOBEC-3C Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 22.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NRW3 (Residues: 1-190; Coverage: 100%)
Gene names: APOBEC1L, APOBEC3C, PBI
Sequence domains: Novel AID APOBEC clade 2

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P61
Unit cell:
a: 105.042Å b: 105.042Å c: 70.046Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.214 0.263
Expression system: Escherichia coli

Quick links

Citations

21 review citations

APOBECs and virus restriction.
Harris et al. (2015)
20 more

36 mentions without citation

The APOBEC Protein Family: United by Structure, Divergent in Function.
Salter et al. (2016)
35 more