› 3vut

X-ray diffraction
3.5Å resolution

Crystal structures of non-phosphorylated MAP2K4

Released:
DOI: 10.2210/pdb3vut/pdb
PDB entry 3vut coloured by chain, front view.
PDB entry 3vut coloured by chain, side view.
PDB entry 3vut coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal and solution structures disclose a putative transient state of mitogen-activated protein kinase kinase 4.
Matsumoto T, Kinoshita T, Kirii Y, Tada T, Yamano A
Biochem Biophys Res Commun 425 195-200 (2012)
PMID: 22828509

Function and Biology Details

Reaction catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155394 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity mitogen-activated protein kinase kinase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 37.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P45985 (Residues: 80-399; Coverage: 80%)
Gene names: JNKK1, MAP2K4, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P212121
Unit cell:
a: 67.773Å b: 87.362Å c: 118.373Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.335 0.331 0.407
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Crystallographic mining of ASK1 regulators to unravel the intricate PPI interfaces for the discovery of small molecule.
Agrahari et al. (2022)
2 other articles

4 mentions without citation

MKK4 Inhibitors-Recent Development Status and Therapeutic Potential.
Katzengruber et al. (2023)
3 more