3vxs

X-ray diffraction
1.8Å resolution

The complex between H27-14 TCR and HLA-A24 bound to HIV-1 Nef134-10(6L) peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-235025 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
H27-14 TCR alpha chain Chain: D
Molecule details ›
Chain: D
Length: 207 amino acids
Theoretical weight: 22.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
H27-14 TCR beta chain Chain: E
Molecule details ›
Chain: E
Length: 244 amino acids
Theoretical weight: 27.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
C-terminal core protein Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.26 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
  • Canonical: Q9WPU2 (Residues: 133-142; Coverage: 5%)
Gene name: nef

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 67.404Å b: 99.047Å c: 162.965Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.23
Expression systems:
  • Escherichia coli
  • Not provided