› 3vyx

X-ray diffraction
2.29Å resolution

Structural insights into RISC assembly facilitated by dsRNA binding domains of human RNA helicase (DHX9)

Released:
DOI: 10.2210/pdb3vyx/pdb
PDB entry 3vyx coloured by chain, front view.
PDB entry 3vyx coloured by chain, side view.
PDB entry 3vyx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into RISC assembly facilitated by dsRNA-binding domains of human RNA helicase A (DHX9).
Fu Q, Yuan YA
Nucleic Acids Res 41 3457-70 (2013)
PMID: 23361462

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170610 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
ATP-dependent RNA helicase A Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 13.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08211 (Residues: 152-264; Coverage: 9%)
Gene names: DDX9, DHX9, LKP, NDH2
Sequence domains: Double-stranded RNA binding motif
Structure domains: Double Stranded RNA Binding Domain
RNA (5'-R(P*GP*CP*GP*CP*GP*CP*GP*CP*GP*C)-3') Chains: B, C
Molecule details ›
Chains: B, C
Length: 10 nucleotides
Theoretical weight: 3.21 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 63.198Å b: 63.198Å c: 107.617Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.263
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

The biology of DHX9 and its potential as a therapeutic target.
Lee et al. (2016)
4 more

11 mentions without citation

'Black sheep' that don't leave the double-stranded RNA-binding domain fold.
Gleghorn et al. (2014)
10 more