› 3vza

X-ray diffraction
1.9Å resolution

Crystal structure of the chicken Spc24-Spc25 globular domain in complex with CENP-T peptide

Released:
DOI: 10.2210/pdb3vza/pdb
PDB entry 3vza coloured by chain, front view.
PDB entry 3vza coloured by chain, side view.
PDB entry 3vza coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
CENP-T provides a structural platform for outer kinetochore assembly.
Nishino T, Rago F, Hori T, Tomii K, Cheeseman IM, Fukagawa T
EMBO J 32 424-36 (2013)
PMID: 23334297

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-236885 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Uncharacterized protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 105 amino acids
Theoretical weight: 12.47 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
Structure domains: Chromosome segregation protein Spc25
Kinetochore protein Spc24 Chains: C, D
Molecule details ›
Chains: C, D
Length: 73 amino acids
Theoretical weight: 8.46 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: R4GRT4 (Residues: 1-73; Coverage: 100%)
Sequence domains: Spc24 subunit of Ndc80
Centromere protein T Chains: E, F
Molecule details ›
Chains: E, F
Length: 39 amino acids
Theoretical weight: 4.42 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: F1NPG5 (Residues: 63-98; Coverage: 6%)
Gene name: CENPT

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P31
Unit cell:
a: 61.321Å b: 61.321Å c: 111.275Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.167 0.216
Expression system: Escherichia coli

Quick links

Citations

34 review citations

The molecular basis for centromere identity and function.
McKinley et al. (2016)
33 more

3 mentions without citation

Physicochemical mechanisms of protein regulation by phosphorylation.
Nishi et al. (2014)
2 more