› 3w0u

X-ray diffraction
1.7Å resolution

human Glyoxalase I with an N-hydroxypyridone inhibitor

Released:
DOI: 10.2210/pdb3w0u/pdb
PDB entry 3w0u coloured by chain, front view.
PDB entry 3w0u coloured by chain, side view.
PDB entry 3w0u coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Fukami TA, Irie M, Matsuura T

Function and Biology Details

Reaction catalysed: 
(R)-S-lactoylglutathione = glutathione + methylglyoxal
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170069 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactoylglutathione lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 187 amino acids
Theoretical weight: 21.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04760 (Residues: 1-184; Coverage: 100%)
Gene name: GLO1
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand HPW 2 x HPW
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21212
Unit cell:
a: 86.17Å b: 67.38Å c: 68.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.242
Expression system: Escherichia coli

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