› 3w5o

X-ray diffraction
2.84Å resolution

Crystal Structure of Human DNA ligase IV

Released:
DOI: 10.2210/pdb3w5o/pdb
PDB entry 3w5o coloured by chain, front view.
PDB entry 3w5o coloured by chain, side view.
PDB entry 3w5o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the catalytic region of DNA ligase IV in complex with an Artemis fragment sheds light on double-strand break repair.
Ochi T, Gu X, Blundell TL
Structure 21 672-9 (2013)
PMID: 23523427

Function and Biology Details

Reaction catalysed: 
(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156091 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 610 amino acids
Theoretical weight: 69.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49917 (Residues: 1-609; Coverage: 67%)
Gene name: LIG4
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand SO4 24 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 68.29Å b: 104.36Å c: 120.04Å
α: 90° β: 94.08° γ: 90°
R-values:
R R work R free
0.196 0.194 0.246
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Non-homologous DNA end joining and alternative pathways to double-strand break repair.
Chang et al. (2017)
10 more

7 mentions without citation

Recognition and repair of chemically heterogeneous structures at DNA ends.
Andres et al. (2015)
6 more