› 3wez

X-ray diffraction
2.11Å resolution

Crystal structure of human beta-galactosidase in complex with NOEV

Released:
DOI: 10.2210/pdb3wez/pdb
PDB entry 3wez coloured by chain, front view.
PDB entry 3wez coloured by chain, side view.
PDB entry 3wez coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Suzuki H, Ohto U, Shimizu T

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147669 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 678 amino acids
Theoretical weight: 76.62 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P16278 (Residues: 24-677; Coverage: 100%)
Gene names: ELNR1, GLB1
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand NAG 16 x NAG
Ligand CL 4 x CL
Ligand N8V 4 x N8V
Ligand SO4 8 x SO4
Ligand EDO 8 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21
Unit cell:
a: 94.933Å b: 115.915Å c: 140.51Å
α: 90° β: 92.25° γ: 90°
R-values:
R R work R free
0.181 0.179 0.225
Expression system: Komagataella pastoris

Quick links