› 3wf3

X-ray diffraction
2.15Å resolution

Crystal structure of human beta-galactosidase mutant I51T in complex with Galactose

Released:
DOI: 10.2210/pdb3wf3/pdb
PDB entry 3wf3 coloured by chain, front view.
PDB entry 3wf3 coloured by chain, side view.
PDB entry 3wf3 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Suzuki H, Ohto U, Shimizu T

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147669 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 678 amino acids
Theoretical weight: 76.61 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P16278 (Residues: 24-677; Coverage: 100%)
Gene names: ELNR1, GLB1
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand NAG 16 x NAG
Ligand GAL 4 x GAL
Ligand CL 4 x CL
Ligand SO4 8 x SO4
Ligand EDO 8 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21
Unit cell:
a: 94.96Å b: 115.888Å c: 140.284Å
α: 90° β: 92.23° γ: 90°
R-values:
R R work R free
0.198 0.196 0.242
Expression system: Komagataella pastoris

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