› 3wh2

X-ray diffraction
1.3Å resolution

Human Mincle in complex with citrate

Released:
DOI: 10.2210/pdb3wh2/pdb
PDB entry 3wh2 coloured by chain, front view.
PDB entry 3wh2 coloured by chain, side view.
PDB entry 3wh2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis for glycolipid recognition by the C-type lectins Mincle and MCL.
Furukawa A, Kamishikiryo J, Mori D, Toyonaga K, Okabe Y, Toji A, Kanda R, Miyake Y, Ose T, Yamasaki S, Maenaka K
Proc Natl Acad Sci U S A 110 17438-43 (2013)
PMID: 24101491

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193945 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C-type lectin domain family 4 member E Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ULY5 (Residues: 74-219; Coverage: 67%)
Gene names: CLEC4E, CLECSF9, MINCLE, UNQ218/PRO244
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P31
Unit cell:
a: 49.213Å b: 49.213Å c: 43.55Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.129 0.128 0.156
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Recent insights into structures and functions of C-type lectins in the immune system.
Drickamer et al. (2015)
21 more

8 mentions without citation

Structural analysis for glycolipid recognition by the C-type lectins Mincle and MCL.
Furukawa et al. (2013)
7 more