3whu

X-ray diffraction
2.6Å resolution

Crystal structure of ERGIC-53/MCFD2, Calcium/Man2-bound form

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-155904 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein ERGIC-53 Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49257 (Residues: 31-269; Coverage: 50%)
Gene names: ERGIC53, F5F8D, LMAN1
Sequence domains: Legume-like lectin family
Structure domains: Jelly Rolls
Multiple coagulation factor deficiency protein 2 Chain: B
Molecule details ›
Chain: B
Length: 104 amino acids
Theoretical weight: 12.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NI22 (Residues: 67-146; Coverage: 67%)
Gene names: MCFD2, SDNSF
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2
Unit cell:
a: 101.68Å b: 58.71Å c: 56.43Å
α: 90° β: 109.68° γ: 90°
R-values:
R R work R free
0.223 0.22 0.273
Expression system: Escherichia coli