› 3wjl

X-ray diffraction
2.86Å resolution

Crystal structure of IIb selective Fc variant, Fc(V12), in complex with FcgRIIb

Released:
DOI: 10.2210/pdb3wjl/pdb
PDB entry 3wjl coloured by chain, front view.
PDB entry 3wjl coloured by chain, side view.
PDB entry 3wjl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineered antibody Fc variant with selectively enhanced FcγRIIb binding over both FcγRIIa(R131) and FcγRIIa(H131).
Mimoto F, Katada H, Kadono S, Igawa T, Kuramochi T, Muraoka M, Wada Y, Haraya K, Miyazaki T, Hattori K
Protein Eng Des Sel 26 589-98 (2013)
PMID: 23744091

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134860 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.01 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 99-328; Coverage: 58%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Low affinity immunoglobulin gamma Fc region receptor II-c Chain: C
Molecule details ›
Chain: C
Length: 179 amino acids
Theoretical weight: 20.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P31995 (Residues: 45-217; Coverage: 62%)
Gene names: CD32, FCG2, FCGR2C, IGFR2
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, FUL
Carbohydrate polymer
1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: C2221
Unit cell:
a: 156.71Å b: 260.2Å c: 56.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.27
Expression system: Homo sapiens

Quick links

Citations

36 review citations

Next generation antibody drugs: pursuit of the 'high-hanging fruit'.
Carter et al. (2018)
35 more

2 mentions without citation

Functional, Biophysical, and Structural Characterization of Human IgG1 and IgG4 Fc Variants with Ablated Immune Functionality.
Tam et al. (2017)
1 more