› 3wn5

X-ray diffraction
2.78Å resolution

Crystal structure of asymmetrically engineered Fc variant in complex with FcgRIIIa

Released:
DOI: 10.2210/pdb3wn5/pdb
PDB entry 3wn5 coloured by chain, front view.
PDB entry 3wn5 coloured by chain, side view.
PDB entry 3wn5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a novel asymmetrically engineered Fc variant with improved affinity for FcγRs.
Mimoto F, Kadono S, Katada H, Igawa T, Kamikawa T, Hattori K
Mol Immunol 58 132-8 (2014)
PMID: 24334029

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134814 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (7 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 230 amino acids
Theoretical weight: 25.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 99-328; Coverage: 58%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Immunoglobulin heavy constant gamma 1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 230 amino acids
Theoretical weight: 26.23 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 99-328; Coverage: 58%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Low affinity immunoglobulin gamma Fc region receptor III-A Chains: C, F
Molecule details ›
Chains: C, F
Length: 197 amino acids
Theoretical weight: 22.63 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P08637 (Residues: 18-208; Coverage: 80%)
Gene names: CD16A, FCG3, FCGR3, FCGR3A, IGFR3
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, FUL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUL
Carbohydrate polymer
2 bound ligands:
Ligand IOD 16 x IOD
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21
Unit cell:
a: 75.03Å b: 72.49Å c: 163.48Å
α: 90° β: 91.15° γ: 90°
R-values:
R R work R free
0.238 0.236 0.274
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Molecular properties of human IgG subclasses and their implications for designing therapeutic monoclonal antibodies against infectious diseases.
Irani et al. (2015)
6 more

2 mentions without citation

Considerations of Antibody Geometric Constraints on NK Cell Antibody Dependent Cellular Cytotoxicity.
Murin CD. (2020)
1 more