› 3wu0

X-ray diffraction
2.6Å resolution

Crystal structure of phosphorylated ETS-1 DNA binding and autoinhibitory domains (276-441)

Released:
DOI: 10.2210/pdb3wu0/pdb
PDB entry 3wu0 coloured by chain, front view.
PDB entry 3wu0 coloured by chain, side view.
PDB entry 3wu0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A novel allosteric mechanism on protein-DNA interactions underlying the phosphorylation-dependent regulation of Ets1 target gene expressions.
Shiina M, Hamada K, Inoue-Bungo T, Shimamura M, Uchiyama A, Baba S, Sato K, Yamamoto M, Ogata K
J Mol Biol 427 1655-69 (2015)
PMID: 25083921

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147155 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein C-ets-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 19.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14921 (Residues: 276-441; Coverage: 38%)
Gene names: ETS1, EWSR2
Sequence domains:
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P31
Unit cell:
a: 56.71Å b: 56.71Å c: 105.984Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.222 0.277
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Studies on Molecular Dynamics of Intrinsically Disordered Proteins and Their Fuzzy Complexes: A Mini-Review.
Kasahara et al. (2019)
16 other articles