› 3wuv

X-ray diffraction
2.79Å resolution

Structure basis of inactivating cell abscission with chimera peptide 2

Released:
DOI: 10.2210/pdb3wuv/pdb
PDB entry 3wuv coloured by chain, front view.
PDB entry 3wuv coloured by chain, side view.
PDB entry 3wuv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and biochemical insights into the role of testis-expressed gene 14 (TEX14) in forming the stable intercellular bridges of germ cells.
Kim HJ, Yoon J, Matsuura A, Na JH, Lee WK, Kim H, Choi JW, Park JE, Park SJ, Kim KT, Chang R, Lee BI, Yu YG, Shin YK, Jeong C, Rhee K, Lee HH
Proc Natl Acad Sci U S A 112 12372-7 (2015)
PMID: 26392564

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176092 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Centrosomal protein of 55 kDa Chains: A, B, D, E, G, H, J, K, M, N, P, Q
Molecule details ›
Chains: A, B, D, E, G, H, J, K, M, N, P, Q
Length: 63 amino acids
Theoretical weight: 7.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q53EZ4 (Residues: 160-217; Coverage: 13%)
Gene names: C10orf3, CEP55, URCC6
Sequence domains: TSG101 and ALIX binding domain of CEP55
Structure domains: Geminin coiled-coil domain
Programmed cell death 6-interacting protein Chains: C, F, I, L, O, R
Molecule details ›
Chains: C, F, I, L, O, R
Length: 14 amino acids
Theoretical weight: 1.54 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q8WUM4 (Residues: 796-809; Coverage: 2%)
Gene names: AIP1, ALIX, KIAA1375, PDCD6IP

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: C2
Unit cell:
a: 233.066Å b: 134.671Å c: 88.325Å
α: 90° β: 90.02° γ: 90°
R-values:
R R work R free
0.202 0.201 0.226
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

New insights into the genetics of spermatogenic failure: a review of the literature.
Cannarella et al. (2019)
3 more

1 mention without citation

Structural and biochemical insights into the role of testis-expressed gene 14 (TEX14) in forming the stable intercellular bridges of germ cells.
Kim et al. (2015)