› 3wv6

X-ray diffraction
1.95Å resolution

Crystal Structure of a protease-resistant mutant form of human galectin-9

Released:
DOI: 10.2210/pdb3wv6/pdb
PDB entry 3wv6 coloured by chain, front view.
PDB entry 3wv6 coloured by chain, side view.
PDB entry 3wv6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray structure of a protease-resistant mutant form of human galectin-9 having two carbohydrate recognition domains with a metal-binding site.
Yoshida H, Nishi N, Wada K, Nakamura T, Hirashima M, Kuwabara N, Kato R, Kamitori S
Biochem Biophys Res Commun 490 1287-1293 (2017)
PMID: 28687490

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Galectin-9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 33.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00182 (Residues: 1-355; Coverage: 83%)
Gene name: LGALS9
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GAL, GLC
Carbohydrate polymer
2 bound ligands:
Ligand ZN 2 x ZN
Ligand EDO 10 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21
Unit cell:
a: 58.34Å b: 94.87Å c: 64.81Å
α: 90° β: 113.87° γ: 90°
R-values:
R R work R free
0.192 0.188 0.222
Expression system: Escherichia coli

Quick links

Citations

1 review citation

A Brief History of Charcot-Leyden Crystal Protein/Galectin-10 Research.
Su J. (2018)
1 other article

2 mentions without citation

The universe of galectin-binding partners and their functions in health and disease.
Troncoso et al. (2023)
1 more