› 3ww0

X-ray diffraction
2.5Å resolution

Crystal structure of F97A mutant, a new nuclear transport receptor of Hsp70

Released:
DOI: 10.2210/pdb3ww0/pdb
PDB entry 3ww0 coloured by chain, front view.
PDB entry 3ww0 coloured by chain, side view.
PDB entry 3ww0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of Hikeshi, a new nuclear transport receptor of Hsp70s.
Song J, Kose S, Watanabe A, Son SY, Choi S, Hong H, Yamashita E, Park IY, Imamoto N, Lee SJ
Acta Crystallogr D Biol Crystallogr 71 473-83 (2015)
PMID: 25760597

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176094 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein Hikeshi Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 21.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53FT3 (Residues: 1-197; Coverage: 100%)
Gene names: C11orf73, HIKESHI, HSPC138, HSPC179, HSPC248
Sequence domains:
Protein Hikeshi Chain: B
Molecule details ›
Chain: B
Length: 198 amino acids
Theoretical weight: 21.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53FT3 (Residues: 1-197; Coverage: 100%)
Gene names: C11orf73, HIKESHI, HSPC138, HSPC179, HSPC248
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P32
Unit cell:
a: 85.483Å b: 85.483Å c: 68.943Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.196 0.224
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural Biology and Regulation of Protein Import into the Nucleus.
Christie et al. (2016)
1 more