› 3wxq

X-ray diffraction
1.6Å resolution

Serial femtosecond X-ray structure of human fatty acid-binding protein type-3 (FABP3) in complex with stearic acid (C18:0) determined using X-ray free-electron laser at SACLA

Released:
DOI: 10.2210/pdb3wxq/pdb
PDB entry 3wxq coloured by chain, front view.
PDB entry 3wxq coloured by chain, side view.
PDB entry 3wxq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Grease matrix as a versatile carrier of proteins for serial crystallography.
Sugahara M, Mizohata E, Nango E, Suzuki M, Tanaka T, Masuda T, Tanaka R, Shimamura T, Tanaka Y, Suno C, Ihara K, Pan D, Kakinouchi K, Sugiyama S, Murata M, Inoue T, Tono K, Song C, Park J, Kameshima T, Hatsui T, Joti Y, Yabashi M, Iwata S
Nat Methods 12 61-3 (2015)
PMID: 25384243

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty acid-binding protein, heart Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 14.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05413 (Residues: 1-133; Coverage: 100%)
Gene names: FABP11, FABP3, MDGI
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand STE 1 x STE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SACLA BEAMLINE BL3
Spacegroup: P212121
Unit cell:
a: 33.707Å b: 54.851Å c: 70.656Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

A Bright Future for Serial Femtosecond Crystallography with XFELs.
Johansson et al. (2017)
8 more

4 mentions without citation

Serial femtosecond crystallography: A revolution in structural biology.
Martin-Garcia et al. (2016)
3 more