› 3wzu

X-ray diffraction
3.01Å resolution

THE STRUCTURE OF MAP2K7 IN COMPLEX WITH 5Z-7-oxozeaenol

Released:
DOI: 10.2210/pdb3wzu/pdb
PDB entry 3wzu coloured by chain, front view.
PDB entry 3wzu coloured by chain, side view.
PDB entry 3wzu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
5Z-7-Oxozeaenol covalently binds to MAP2K7 at Cys218 in an unprecedented manner.
Sogabe Y, Matsumoto T, Hashimoto T, Kirii Y, Sawa M, Kinoshita T
Bioorg Med Chem Lett 25 593-6 (2015)
PMID: 25529738

Function and Biology Details

Reaction catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity mitogen-activated protein kinase kinase 7 Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14733 (Residues: 103-419; Coverage: 76%)
Gene names: JNKK2, MAP2K7, MEK7, MKK7, PRKMK7, SKK4
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 1FM 1 x 1FM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P6122
Unit cell:
a: 71.395Å b: 71.395Å c: 268.817Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.236 0.322
Expression system: Escherichia coli

Quick links

Citations

4 review citations

JNK Signaling: Regulation and Functions Based on Complex Protein-Protein Partnerships.
Zeke et al. (2016)
3 more

1 mention without citation

A Chemical Probe Strategy for Interrogating Inhibitor Selectivity Across the MEK Kinase Family.
Deibler et al. (2017)