› 3zdl

X-ray diffraction
2.3Å resolution

Vinculin head (1-258) in complex with a RIAM fragment

Released:
DOI: 10.2210/pdb3zdl/pdb
PDB entry 3zdl coloured by chain, front view.
PDB entry 3zdl coloured by chain, side view.
PDB entry 3zdl coloured by chain, top view.
Source organisms:
Primary publication:
RIAM and vinculin binding to talin are mutually exclusive and regulate adhesion assembly and turnover.
Goult BT, Zacharchenko T, Bate N, Tsang R, Hey F, Gingras AR, Elliott PR, Roberts GCK, Ballestrem C, Critchley DR, Barsukov IL
J Biol Chem 288 8238-8249 (2013)
PMID: 23389036

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146034 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vinculin Chain: A
Molecule details ›
Chain: A
Length: 280 amino acids
Theoretical weight: 31.33 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12003 (Residues: 1-259; Coverage: 23%)
Gene names: VCL, VINC1
Sequence domains: Vinculin family
Structure domains: Alpha-catenin/vinculin-like
Amyloid beta A4 precursor protein-binding family B member 1-interacting protein Chain: B
Molecule details ›
Chain: B
Length: 32 amino acids
Theoretical weight: 3.54 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q7Z5R6 (Residues: 1-32; Coverage: 5%)
Gene names: APBB1IP, PREL1, RARP1, RIAM

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P21212
Unit cell:
a: 51.73Å b: 70.03Å c: 95.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.23
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

33 review citations

Talins and kindlins: partners in integrin-mediated adhesion.
Calderwood et al. (2013)
32 more

3 mentions without citation

RIAM and vinculin binding to talin are mutually exclusive and regulate adhesion assembly and turnover.
Goult et al. (2013)
2 more