› 3zfy

X-ray diffraction
2.2Å resolution

Crystal structure of EphB3

Released:
DOI: 10.2210/pdb3zfy/pdb
PDB entry 3zfy coloured by chain, front view.
PDB entry 3zfy coloured by chain, side view.
PDB entry 3zfy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Completing the structural family portrait of the human EphB tyrosine kinase domains.
Overman RC, Debreczeni JE, Truman CM, McAlister MS, Attwood TK
Protein Sci 23 627-38 (2014)
PMID: 24677421

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ephrin type-B receptor 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 33.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54753 (Residues: 616-910; Coverage: 31%)
Gene names: EPHB3, ETK2, HEK2, TYRO6
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P1
Unit cell:
a: 46.99Å b: 56.488Å c: 61.354Å
α: 93.02° β: 90.65° γ: 90.03°
R-values:
R R work R free
0.226 0.224 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Factors involved in heterologous expression of proteins in E. coli host.
Pouresmaeil et al. (2023)
9 other articles

3 mentions without citation

Completing the structural family portrait of the human EphB tyrosine kinase domains.
Overman et al. (2014)
2 more